Concurrent mRNA and protein extraction from the same experimental sample using a commercially available column-based RNA preparation kit

Concurrent mRNA and protein extraction from the same experimental sample using a commercially available column-based RNA preparation kit.

Column-based method to simultaneously extract DNA, RNA, and proteins from the same sample.

We describe a procedure for the simultaneous extraction of proteins and nucleic acids from the same experimental sample allowing for direct correlations between genetic, genomic, and proteomic data. This approach, using commercially available column-based nucleic acid extraction kits, requires no hazardous chemicals and is a quick, reliable, and consistent method for concomitant protein extract...

Comparison of commercially-available RNA extraction methods for effective bacterial RNA isolation from milk spiked samples

Nucleic-acid based methods for bacterial identification are extremely useful in diagnostic applications due to their specificity and sensitivity. However, they require an optimal purification of the target molecules. As part of the development of a new diagnostic method for the detection of bacterial RNA in cow milk, we have compared four commercially available RNA extraction kits for the isola...

A Simple Complexation Model and the Experimental Datafor Protein Extraction Using Reverse Micellar Systems

Concurrent Sweet’s syndrome and erythema nodosum: two manifestations of the same disease or the same spectrum?

Sweet’s syndrome and erythema nodosum are rarely seen together. Herein, we report a case of concurrent Sweet’s syndrome and erythema nodosum and review previous cases.